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KMID : 0545120100200020370
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 2 p.370 ~ p.374
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Cloning of aprE86-1 Gene Encoding 27 kDa Mature Fibrinolytic Enzyme from Bacillus amyloliquefaciens CH86-1
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Lee Ae-Ran
Kim Gyoung-Min
Kwon Gun-Hee
Lee Kang-Wook
Park Jae-Yong
Chun Ji-Yeon
Cha Jae-Ho
Song Young-Sun
Kim Jeong-Hwan
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Abstract
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A gene, encoding the major secreted fibrinolytic protein of Bacillus amyloliquefaciens CH86-1, was cloned from the genomic DNA. DNA sequencing showed that the gene, aprE86-1, could direct the synthesis of a mature protein of 275 amino acids long after processing. When aprE86-1 was introduced into B. subtilis, 27 kDa mature protein was produced as expected. The fibrinolytic activity of B. subtilis transformant (TF) was higher than that of B. amyloliquefaciens CH86-1, showing the possibility of increasing fibrinolytic activities of Bacillus strains through genetic engineering.
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KEYWORD
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Bacillus amyloliquefaciens, fibrinolytic enzymes, gene expression
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